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FEE NOTICE: Mouse Biology Program is in the process of an across the board rate review, affecting MBP, MMRRC at UC Davis, MMPC-Live at UC Davis, GMRC, and MBSR. Our rates will be updated as early as October 1, 2023. Orders submitted prior to that date will not be affected, however fees quoted but no order request submitted will be subject to the new rates. Our per diem rates for all MBP housed and managed colonies will be billed the new rates starting October 23rd. We will release a copy of the new rates as soon as they are approved.

Protocols

Please visit UC Davis MMPC-Live Catalog for products and protocols at the Mouse Metabolic Phenotyping Center at UC Davis.

Services

Animal Care and Housing
Cryo Storage
Embryo Targeting and Transgenics
ES Cell Work
Informatics
MBP Products
Molecular Biology
Patient and Pet Derived Xenograft Services
Phenotyping Services
Project Management and Import/Export Services
Reproductive Technologies and Cryopreservation

For current pricing, please inquire at mbp@ucdavis.edu.

Animal Care and Housing
Service	Description
Special Service – Animal Tech	Special services by Animal Technician staff, hourly rate
Special Service – SRA3	Advanced or Surgical Services, hourly rate
Professional Services	DVM hourly rate
Colony Management	Colony management including breeding, weaning, sampling, monitoring, per cage per day; does not include research data collection or management, injections, time mated pregnancy, or other misc services.
Small Crate Packing	Sterilized small mouse crate, 1 – 2 sections, including food, gel, preparation and packing
Large Crate Packing	Sterilized large mouse crate, 1 – 4 sections, including food, gel, preparation and packing
Intercampus Cage Packing	Packing and perparation of up to 5 cages for UCD campus transport
Duplex Bubble Biweekly Maint.	Biweekly maintenance fee for a duplex size single isolator bubble to cover the cost for daily animal checks, weekly cage cleanings if needed and biweekly isolator entry/exit for movement of supplies in and out of isolator. This is in place of a cage per diem fee. This fee does not include any pathogen screening, sample collection, or breeding management, as these will be project dependent and charged according to project needs.
Quad Bubble Biweekly Maint.	Biweekly maintenance fee for a quad size single isolator bubble to cover the cost for daily animal checks, weekly cage cleanings if needed and biweekly isolator entry/exit for movement of supplies in and out of isolator. This is in place of a cage per diem fee. This fee does not include any pathogen screening, sample collection, or breeding management, as these will be project dependent and charged separately according to project needs.
Isolator Setup – 1 Bubble	Set-up costs of a gnotobioic isolator for the specified number of separate bubbles. This fee includes the 1 month validation including pahtology screening. No mice are present in the isolator during this setup validation phase. Bubble setup supplies includes liquid steraliants, filter media, tape, wypalls. Startup Mouse Maintenances supplies includes mold trap, gloves, forceps, bands, port cap, nalgene, filter media, laundry bag, misc.
Isolator Setup – 2 Bubble	Set-up costs of a gnotobioic isolator for the specified number of separate bubbles. This fee includes the 1 month validation including pahtology screening. No mice are present in the isolator during this setup validation phase. Bubble setup supplies includes liquid steraliants, filter media, tape, wypalls. Startup Mouse Maintenances supplies includes mold trap, gloves, forceps, bands, port cap, nalgene, filter media, laundry bag, misc.
Isolator Setup – 3 Bubble	Set-up costs of a gnotobioic isolator for the specified number of separate bubbles. This fee includes the 1 month validation including pahtology screening. No mice are present in the isolator during this setup validation phase. Bubble setup supplies includes liquid steraliants, filter media, tape, wypalls. Startup Mouse Maintenances supplies includes mold trap, gloves, forceps, bands, port cap, nalgene, filter media, laundry bag, misc.
Isolator Setup – 4 Bubble	Set-up costs of a gnotobioic isolator for the specified number of separate bubbles. This fee includes the 1 month validation including pahtology screening. No mice are present in the isolator during this setup validation phase. Bubble setup supplies includes liquid steraliants, filter media, tape, wypalls. Startup Mouse Maintenances supplies includes mold trap, gloves, forceps, bands, port cap, nalgene, filter media, laundry bag, misc.
Immunodeficient CM	For immunodeficient mice that are handled with extra PPE and stricter practices.
Gnoto Special Service	Technical staff hourly service fee.
Disposable Caging Per Diem	Housing in disposable cages (e.g. BSL2 studies), 1 – 5 mice per cage; Includes cost of disposable cage for a 2wk cage change and additional PPE
Disposable Cage	Disposable caging, primarily used for BSL2, chemotheraputics, or similar projects
Delivery/Vehicle Charge	Charge Delivery of items to/from campus, including use of Vivarium vehicle
Cylinder Fee	Supply and personnel costs to prepare a cylinder with supplies and autoclave the packed isolator.
Port Entry/Exit	Supply costs and personnel time to physically enter/exit a cylinder through the port to bring in and/or remove supplies.
Conventional Housing Per Diem	Housing of mice in a conventional facility, 1 – 5 mice per cage, per cage per day
Barrier Housing Per Diem	Housing of mice in a barrier facility, 1 – 4 mice per cage, per cage per day
BALB/c Germ-Free Mice	Cost for a single germ-free mouse on a BALB/c background. (Campus use only)
B6 Germ-Free Mice	Cost for a single germ-free mouse on a B6 background. (Campus use only)
Animal order fee	Vendor or ATS transfer order placement
2nd Street Pseudopregnant Animal	Pseudopregnant ICR/CD1 mouse derived at 2nd street vivarium (Campus use only)
M3 Pseudopregnant Animal	Pseudopregnant ICR/CD1 mouse derived in M3 vivarium (Campus use only)
NSG Bulk	NSG per mouse fee, quantities of 100+ ordered per month, requires special arrangements with MBP (Campus use only)
NSG	NSG per mouse fee, quantities of <99 ordered per month (Campus use only)
M3 CD-1	ICR/CD1 mouse from barrier facility (Campus use only)
M3 B6	C57BL/6 mouse from barrier facility (Campus use only)
2nd B6 6 -9 wks	C57BL/6 mouse from conventional facility, 6 – 9 weeks of age (Campus use only)
2nd B6 3-5 wks old	C57BL/6 mouse from conventional facility, 3 – 5 weeks of age (Campus use only)
Swiss Webster Germ-Free Mice	Cost for a single germ-free mouse on a Swiss Webster background. (Campus use only)

Cryo Storage
Service	Description
Cryo Storage Fees – 1 year	One year prepaid of cryopreservation storage for one mouse strain (up to 20 embryo straws and/or 30 sperm vials).
Cryo Storage Fees – 3 years	Three years prepaid of cryopreservation storage for one mouse strain (up to 20 embryo straws and/or 30 sperm vials).
Cryo Storage Fees – 5 years	Five years prepaid of cryopreservation storage for one mouse strain (up to 20 embryo straws and/or 30 sperm vials).

Embryo Targeting and Transgenics
Service	Description
SPECIAL SERVICE (MTGL)	Hourly rate for one-off services by the microinjection technical staff
Basic DNA Pronuclear Injection (Hybrid)	Includes injection of upto 180 and transfer of upto 120 injected zygotes, or obtaining 35 live pups, or generating 2 founders, whichever comes first. (B6D2F1)
Basic DNA Pronuclear Injection (Inbred)	Includes injection of up to 180 and transfer of up to 120 injected zygotes, or obtaining 35 live pups, or generating 2 founders, whichever comes first. Inbred donors (B6, FVB)
Basic ESC Injection – Inbred (up to 3 clones)	Inject up to 3x ES Cell cones per gene, inject 30-40 inbred donor embryos per clone, embryo transfer into up to 6 recipients, derive at least 10 pups, and house pups until 7 weeks of age. There are no guarantees for derivation of high percent chimeric mice with basic microinjection services.
Basic ESC Injection – Outbred (up to 3 clones)	Inject up to 3x ES Cell cones per gene, inject 30-40 outbred donor embryos per clone, embryo transfer into up to 6 recipients, derive at least 10 pups, and house pups until 7 weeks of age. There are no guarantees for derivation of high percent chimeric mice with basic microinjection services.
Basic ESC Injection-Inbred (1 clone)	Inject one ES Cell cone to 30-40 donor embryos, embryo transfer into 2 recipients, derive at least one litter of pups, and house pups until 7 weeks of age. There are no guarantees for derivation of high percent chimeric mice with basic microinjection services.
CRISPR Advanced Targeting	This CRISPR service includes microinjection or electroporation of up to 160 fertilized zygotes (B6, FVB), and embryo transfers into 6 – 8 pseudopregnant recipients, and housing of born mice for up to 10 weeks. Service is for SNP, Homology Directed Repair, or Homologous Recombination, and is intended to generate at least 1 genotypically confirmed founder.
CRISPR Basic Targeting	This CRISPR service includes microinjection or electroporation of up to 100 fertilized zygotes (B6, FVB), and embryo transfers in upto 4 pseudopregnant recipients, and housing of born mice for up to 10 weeks. Service is for InDels or ExDels, and is intended to generate at least 1 genotypically confirmed founder.

ES Cell work
Service	Description
Special Service (MSCL)	ES Cell special handling or technician services, hourly rate
Electroporation	Per target, electroporation, picking up to 3 x 96 well plates, selection, passing, freezing, expansion of 12 clones, mycoplasma testing up to 4 clones, preparing up to 3 for injections
CRISPR ES cell targeting	Per target, crispr es cell targeting, picking 1 x 96 well plate, selection, passing, freezing, expansion of 12 clones, mycoplasma testing up to 4 clones, preparing up to 3 for injections
Recombinase Electroporation (Flp and CRE)	Electroporation of a positive clone with recombinase (CRE or FLP), includes feeder preparation, passing picking, feeding, expansion of up to 12 clones, mycoplasma testing of up to 4 clones, preparing 3 clones for injections
Clone Expansion	Expansion of an ES Cell clone from one to many vials, including feeders if needed, freezing down, and mycoplasma testing
Chromosome Counting	Preparing cells and counting 20 spreads to derive % euploidy
Subcloning	Picking 1 X 96 plates, expansion of up to 12 clones and freezing down, prepping for BL injection, chromosome counting of 6 clones

Informatics
Service	Description
BioAnalytics	BioAnalystics, such as genomic sequence data, hourly rate.
Professional Consultation	Informatics Consultation, Managerial level, hourly rate
Programming	Programmer, hourly rate
Sytem Administration – Special Service	System Administrator, hourly rate
Data Storage – Basic, per image	Includes storage and backup of data, able to retrieved by client for up to 30 days.
Data Storage – Premium, per image	Includes storage and backup of image data, able to be retrieved by client for up to 5 years. Over the 5 years, system administrators will be providing data backup, server administration, system analysis, and checks.
Data Retrieval – per image	Includes retrieval of scanned images if required beyond the requested basic storage time (30 days).

MBP Products
Service	Description
First Mutant ES Cell Clone	Clone purchase, expansion, assessment, genotyping protocol development (PCR, qPCR, and/or LRPCR) and analysis, pathogen testing, 1 vial
Additional ES Cell Clone, same Mutation	Clone purchase, expansion, assessment, genotype analysis, pathogen testing (protocols already developed with first clone), 1 vial
Ultra Low Passage Parental ES Cells	High quality cells at ultra low passage for creating multiple targeted genes, 1 vial
Low Passage Parental ES Cells	High quality cells at low passage for targeting one gene, 1 vial
High Passage Control ES Cells	Quality cells at high passage suitable for DNA analysis, wild type controls, non-targeting use, 1 vial
Irradiated Feeder Cells for ES Cell Culture	One vial of feeder cells used for plating and growing ES cells on, the cells are irradiated prior to expansion and use, and are usually purchased in conjunction with other products.
Live Mutant Mouse	Per live mutant mouse, generally breeder age, and genotyped.
Cryopreserved Sperm	One aliquot of sperm sufficient for fertilizing multiple clutches of oocytes.
Cryopreserved Embryos	One aliquot of embryos, generally 20 – 25 embryos, sufficient for transfer to 1 or 2 embryo recipients
Guaranteed Embryos	One aliquot of embryos, generally 20 – 25 embryos, sufficient for transfer to 1 or 2 embryo recipients, if your recovery is not successful MBP will recover the line for you.

Molecular Biology
Service	Description
Special Services (MGEL)	Hourly rate for one-off services by the molecular biology technical staff
SNP Speed Congenics	Per sample SNP analysis, for confirming % desired strain
Loss of Allele (LOA) ES cell Screening	loss of native allele analysis for ES Cell plate screening, per 96-well plate
PCR SEQUENCING	Sequencing of a PCR product, per sample, includes PCR
RACE Procedure	Rapid amplification of cDNA ends, per clone, generally for confirming gene traps
QPCR Single Reaction/sample	quantitative PCR analysis, does not detect zygosity
QPCR Double Rxn/sample	quantitative PCR analysis, double reaction for determining zygosity or copy number
QPCR Development (per probe)	Development of quantitative PCR, includes analysis, design, and ordering one probe, as well as validation and optimization
Protocol Incorporation	Incorporation of an existing PCR protocol, per allele, including ordering primers and reagents
Standard PCR (per sample)	Per sample cost for testing samples by PCR, does not include protocol development or incorporation if MBP has not yet run this protocol
Standard PCR Development	Development of a standard PCR protocol, per allele, including analysis, design, and ordering Primers, as well as validation and optimization
Southern Blot	Service is per targeting arm–up to 18 samples with the same NEO probe (expansion of ES cells not included)
CRISPR gRNA Design	Design of CRISPR guides for InDel or ExDel services
CRISPR Insertion or Deletion guide construction	Synthesis of CRISPR guides for InDel or ExDel services
CRISPR HDR guide & Oligo or SNP construction	Design and synthesis of CRISPR guides and small oligos or SNPs
CRISPR guide and up to 3 kb DNA synthesis	Design and synthesis of CRISPR guides and DNA of up to 3kb
CRISPR guide and 3 – 5 kb DNA synthesis	Design and synthesis of CRISPR guides and DNA of 3kb up to 5kb
CRISPR guide and 5 – 7 kb DNA synthesis	Design and synthesis of CRISPR guides and DNA of5kb up to 7kb
CRISPR guide and 7 – 9 kb DNA synthesis	Design and synthesis of CRISPR guides and DNA of 7kb up to 9kb
CRISPR gRNA QC	Quality control and assurance analysis of CRISPR guides
CRISPR Prep	Prep of CRISPR reagents for microinjection
CRISPR Off-Target Analysis	Sequence analysis of potential off target effects from CRISPR targeting, per predicted locus, up to 4 samples.
Taqman Gene Expression (per tissue)	Analysis of tissue for expected transgene expression
DNA Vector Design.QC	Quality control analysis of DNA vectors, and design review
Full Vector Purification and Prep	Purification and linearization of vectors used for targeting or transgenesis
Final Vector Prep	Preparation of vector for electorporation or microinjection

Patient and Pet Derived Xenograft services (PDX/PetDX)
Service	Description
PDX/PetDX Tumor Monthly Monitoring 1-5 Mice	Includes monthly monitoring of body weights and measuring of the mice and tumors, medical waste, per each following month.
PDX/PetDX Tumor Implant – 1-5 Mice	Includes supplies (anesthesia, media, and consumables) and labor for surgical implant into 1 – 5 mice, first month monitoring, body weights and measuring of the mice and tumors for the first month (2 – 3 times weekly dependent on growth rate, medical waste collections, collection of tumor for passage or freezing, and transfer of tumor to Med center or other.
PDX/PetDX Tumor Implant – PDX 1-5 Mice CC Member	Includes supplies (anesthesia, media, and consumables) and labor for surgical implant into 1 – 5 mice, first month monitoring, body weights and measuring of the mice and tumors for the first month (2 – 3 times weekly dependent on growth rate, medical waste collections, collection of tumor for passage or freezing, and transfer of tumor to Med center or other; specific to UCD Comprehensive Cancer Center Members.
PDX/PetDX Tumor Monthly Monitoring 1-5 Mice CC Member	Includes monthly monitoring of body weights and measuring of the mice and tumors, medical waste, per each following month; specific to UCD Comprehensive Cancer Center Members.

Phenotyping Services
Service	Description
SPECIAL SERVICES IN-LIFE	Hourly rate for one-off services by an in vivo phenotyping technician
SHIRPA	Standardized procedures for testing cerebellar, muscle, sensory and neuropsychiatric functions, per mouse.
PHYSICAL EXAM	Gross exam per mouse.
ATAXIA (TREADSCAN)	Per mouse treadscan and basic raw data analysis.
OPEN FIELD	1 – 8 mice per run, cost is per run.
ACOUSTIC STARTLE	per run, up to 6 mice per run
MORRIS WATER MAZE (FEMALE)	up to 16 mice over 5 days, females have additional analysis on day 5
MORRIS WATER MAZE (MALE)	up to 16 mice over 5 days
RADIAL ARM MAZE	up to 16 mice over 10 days
INJECTIONS (IP,SUBQ,PO,IM)	Dosing with injectible or oral medication or treatment
FOOD OR WATER INTAKE	per cage, often one mouse per cage
BODY WEIGHT	per mouse
Y-MAZE	per mouse
BLOOD COLLECTIONS (SUBMANDIBULAR,SAPHENOUS VEIN)	includes processing for serum or plasma, cost is per mouse
SOCIAL NOVELTY	testing is over 2 days, cost is per mouse
PASSIVE AVOIDANCE	testing is over 3 days, cost is per mouse
PROJECT MANAGEMENT	Phenotyping specialist, project management and consulting, hourly rate
ACCELERATING ROTAROD	per mouse
X-RAY	Xray or DEXA imaging per mouse, includes anesthesia
Special Service After-life	Ex vivo services, hourly rate for one-off technician services
Blood Collection (Cardiac)	Cardiac blood collection, terminal, per mouse
Basic Necropsy	Basic necropsy includes collection of major organs and snap freezing or PFA or Formalin fixing, fee is per mouse
Advanced Necropsy	Advanced necropsy includes anything outside of the basic necropsy and/or special fixative procedures, major organs can also be a part of this necropsy, per mouse fee
LacZ Whole Mount	Perfusion, specialized necropsy, and organ whole-mount staining for LacZ (per mouse), basic annotation
Scanner Set-up (up to 15 slides)	Set up scanner for slide imaging, including slide prep, and data entry, up to 15 slides per set-up.
Sitting Fee	Hourly reservation, operation, support and maintenance of equipment.

Project Management and Import/Export Services
Service	Description
Special Services (MRPM)	Hourly rate for one-off services by a Project manager or repository technician
Domestic Packaging and Handling	28 x 23 x 18cm Carton and insulated container, up to 3kg of dry ice, or ice packs, retrieving, packing and preparing for shipment
International Packaging and Handling	35.6 x 35.6 x 25.4cm Carton and insulated container, up to 10kg dry ice or ice packs, retrieving, packing and preparing for shipment
Order Processing	Order initiation, including preparation of estimate, processing payment details, use agreements, billing service, hourly rate
Strain/Project Management	Project management services, including strain breeding management, ensuring project flow, timely completion, deliverables me
Importation	Coordinating importation of mice, germplasm, tissue, or cells from outside institutions or companies to UC Davis campus
Exportation	Coordination of exportation of mice, germplasm, tissue, or cells to outside institutions from UC Davis campus
Curation	Strain curation for cataloging and publication purposes
Shipping, Courier Fees	Pass through of courier fees if we use our courier account
Additional Handling	Extra documentation preparation or other special handling of shipments
Vapor Shipper Preparation and Handling	Fill and charge the nitrogen dry shippers, preparing it for a maximum hold time, including assurance of routine QC of our MBP shippers
Contract or License Processing fee	Processing business contracts, KOMP license payments, other agreements handling documents, communications and followup with campus, accounts payables, and IP agents.

Reproductive Technologies and Cryopreservation
Service	Description
SPECIAL SERVICE (MICL)	Hourly rate for one-off services by the reproductive sciences technical staff
ICSI RESCUE	Intracytoplasmic sperm injection (ICSI) for rescue of a mouse line, assisted in vitro fertilization (IVF), implant of embryos into pseudopregnant recipients into the mouse barrier facility, to live birth of pups, includes up to 2 procedures, house litter(s) up to 7 weeks of age, sample pups for genotyping purposes, health screening of recipient females, and provision of health report after weaning.
IVF KIT	Optimized media and reagents for performing in vitro fertilization (IVF), including protocols.
EMBRYO CRYO OF EXTRA EMBRYOS	Cryopreservation of excess mouse embryos derived from rederivation or recovery procedures not needed for the embryo transfers.
EMBRYO CRYOPRESERVATION	Cryopreservation of mouse embryos derived from IVF, including donor female purchase (standard strains such as B6/J, FVB, B6/N, CD1) for Heterozygous line, includes up to two IVF procedures, cryoarchiving up to 300 embryos. 1 year of storage fee included. Homozygous line fees will vary, and may require additional breeding and cryo sessions.
EMBRYO RECOVERY	Recovery of cryopreserved embryos, implant of embryos into pseudopregnant recipients into the mouse barrier facility, to live birth of pups, includes up to 2 procedures, house litter(s) up to 6 weeks of age, sample pups for genotyping purposes, health screening of recipient females, and provision of health report after weaning.
EMBRYO TEST THAW TO PUPS	Thaw of one straw of embryos and implant to pseudopregnant female, derive live births, count % recovered, % born. Not done in barrier, can be sampled for genotyping if requested, recipient can be submitted for health screening if requested at additional costs. Pups are euthanized prior to weaning.
EMBRYO TEST THAW-CULTURE	Thaw of one straw of embryos and culture to blastocyst stage, count % recovered and % developed.
SPERM COUNTS+PRODUCTION	Testes and epididymus collection from 2 males, epididymal sperm count measurements, including tissue weights, homogenizations, counting sperm cells, and calculations. Sperm production measurements including weighing tissues, homogenizations, count spermatids and sperm cells, and calculations. Provcision of a formal report on the assessment.
Sperm Cryo Kit	Optimized media and reagents for performing sperm cryopreservation, including protocols.
SPERM CRYOPRESERVATION	Cryopreservation of mouse sperm from up to 2 male mice.
SPERM MOTILITY-MORPHOLOGY	Collection and assessment of sperm morphology, including digital sperm photography, sperm motility, and a formal report on the assessments. 2 male mice per service.
SPERM RECOVERY	Cryorecovery of mouse sperm, including IVF procedures, implant of embryos into pseudopregnant recipients into the mouse barrier facility, to live birth of pups, includes up to 2 procedures, house litter(s) up to 7 weeks of age, sample pups for genotyping purposes, health screening of recipient females, and provision of health report after weaning.
SPERM TEST THAW TO CULTURE	Cryorecovery of mouse sperm, including IVF procedures and culture to blastocyst stage, count % 2 cell/total oocytes and % of 2 cell developed to blastocysts.
SPERM TEST THAW TO PUPS	Cryorecovery of mouse sperm, including IVF procedures, implant to pseudopregnant female, derive live births, count % 2-cell/total oocytes, and % of implanted born, can be sampled for genotyping if requested, recipient can be submitted for health screening if requested at additional costs. Pups are euthanized prior to weaning.
STRAIN REDERIVATION	Rederivation of a mouse line from fresh spermincluding IVF procedures, implant of embryos into pseudopregnant recipients into the mouse barrier facility, to live birth of pups, includes up to 2 procedures, house litter(s) up to 7 weeks of age, sample pups for genotyping purposes, health screening of recipient females, and provision of health report after weaning.

Place An Order Now

The University of California, Davis, Mouse Biology Program (MBP) is a coordinated, campus-supported teaching, research, and service resource of infrastructure and fundamental expertise directed at developing, promoting, and enhancing the use of mouse model systems for understanding and resolving biological problems. Service rates listed here are university approved and subject to change.

If eligible, UC Davis Cancer Center members may receive a discount on Cancer related research.

Prices listed subject to change (last update 02/07/2019). Request a quote from MBP Customer Service (mbp@ucdavis.edu) for your project.

* Payment must be made from a UC account. Shipping, order processing, and crate or box fees are extra.

** Invoices will be emailed to the client. Shipping, order processing and crate or box fees are extra.