Innovative Tools and Techniques for Advancing Mouse Model Research with help from the Mouse Biology Program.
The Mouse Biology Program (MBP) utilizes a wide range of advanced technologies to support creating, phenotyping, and managing genetically modified mouse models. These technologies ensure precise genetic modifications, comprehensive analysis, and effective colony management, enabling groundbreaking research across various disease areas.
Genetic Engineering Technologies
Reproductive Technologies
Cryopreservation Technologies
Phenotyping Technologies
Health and Colony Management Technologies
Genetic Engineering Technologies
CRISPR/Cas9 Genome Editing: A powerful tool for making targeted gene edits, enabling quick creation of knockout, knockin, and conditional models.
Embryonic Stem (ES) Cell Technology: Used for precise genetic modifications, including large DNA insertions, targeted gene replacements, and conditional alleles.
Pronuclear Injection: A method for generating transgenic mice by inserting DNA into zygotes, supporting random DNA integration and overexpression studies.
Electroporation: Utilized for introducing DNA into cells, including embryonic stem cells, during genetic modification processes.
Reproductive Technologies
In Vitro Fertilization (IVF): Allows for rapid expansion of mouse colonies or recovery of strains from cryopreserved sperm samples.
Embryo Transfer: Used for creating pathogen-free lines or retrieving mouse strains from genetically modified embryos.
Sperm Sieve Technology: A specialized tool for separating motile sperm, enhancing the success of IVF and other reproductive applications.
Cryopreservation Technologies
Embryo Cryopreservation: Ensures long-term storage of mouse strains, preserving valuable genetic lines for future use.
Sperm Cryopreservation: A cost-effective option for maintaining genetic material, supporting efficient strain recovery and disaster recovery planning.
Cryorecovery: Technologies for thawing and reviving cryopreserved embryos or sperm, enabling rapid restoration of mouse strains.
Phenotyping Technologies
Metabolic Chamber Systems: Used to measure energy expenditure, food and water intake, and metabolic rate in mouse models of metabolic diseases.
Behavioral Testing Platforms: Specialized setups for assessing neurological function, cognitive behavior, and social interactions in mouse models.
Cardiovascular Monitoring: Non-invasive systems that measure blood pressure, heart rate, and vascular function in cardiovascular models.
Immunological Assays: Tools for evaluating immune responses and inflammatory processes in mouse models of autoimmune diseases.
Health and Colony Management Technologies
Automated Genotyping Platforms: Rapidly confirm genetic modifications and track genotypes, ensuring accuracy in colony management.
Pathogen Surveillance Systems: Advanced diagnostic tools to monitor and maintain pathogen-free colonies, preserving the health of mouse models.
Colony Management Software: Digital tools that track breeding, lineage, and genotyping, streamlining the management of large mouse colonies.
Driving Innovation with Advanced Technologies
We employ these cutting-edge technologies at MBP to deliver high-quality mouse models, comprehensive phenotyping, and effective colony management. Each technology supports researchers’ efforts to advance biomedical science and improve human health.
