Molecular Construct
Create, synthesize, and validate coding and non-coding gene sequences, ensuring seamless, precise integration into the mouse genome.
Advanced molecular biology techniques create precise constructs for various allele types, including Knockout, knockin, Transgenic, Conditional, and COIN models. Our approach, which uses traditional restriction site cloning, BAC recombineering, Gibson Assembly, and complete gene synthesis, ensures flexibility and precision in construct design.
Our standard vector construction service includes the following:
- Project Consultation: Expert guidance to review project goals and recommend the optimal design and approach for creating the desired molecular construct.
- Informatics Review: Verification of exon/intron structures, confirmation of nucleotide sequences, and integration of specialized cassettes, such as selectable markers or reporter genes.
- Plasmid DNA Purification: High-yield plasmid DNA purification, exceeding requirements for transfection or electroporation into embryonic stem (ES) cells or zygotes.
- Genetic Validation: Complete Sanger or next-generation sequencing (NGS) to confirm an error-free construct.
- Construct Schematic: A publication- or grant-ready schematic for use in research papers, presentations, or funding applications.
Additional services are available upon request to support your research further:
- Detailed Methods Section: Comprehensive documentation tailored for publications or reports.
- Glycerol Stock: Includes the final plasmid and/or parent BAC clone for long-term storage.
- Gel Images: Verification images from PCR and restriction digestion steps.
These comprehensive services ensure the creation of high-quality, fully validated molecular constructs tailored to your research objectives, providing all the tools needed to advance your studies confidently.
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