At the heart of the Mouse Biology Program are research and education. Below is our digital resource library, where we host links and downloads about our published research, available services, and educational material.
MBP Research
Conditional targeting of Ispd using paired Cas9 nickase and a single DNA template in mice
Angus Yiu-fai Lee and Kevin C. Kent Lloyd
This study injected Cas9 RNA and sgRNAs with a single DNA template encoding two loxP sites flanking (floxing) exon 2 of the isoprenoid synthase-containing domain (Ispd) into the pronucleus and cytoplasm of C57BL/6NCr one-cell stage zygotes. After surgical transfer, one F0 mouse expressing a conditional allele was produced (at a frequency of ∼8% of live pups born). The floxed allele was transmitted through the germline to F1 progeny and could be successfully recombined using Cre recombinase. This study indicates that conditional targeting can be accomplished effectively using paired Cas9n and a single DNA template.
Rescue of germline transmission from chimeras by IVF after sperm analysis
Li MW, Willis BJ, Evans KD, Araiza RS, Lee AY, Lloyd KC.
In this study, we assessed the production, quality, and genotype of epididymal sperm harvested from male chimeras generated by blastocyst or morula microinjection of gene-targeted embryonic stem (ES) cell clones containing a LacZ expression cassette and that failed to achieve GLT. Our results indicate that most chimeras that do not achieve GLT by natural mating are infertile, and the primary cause of infertility is failed spermatogenesis. We conclude that an assessment of coat color chimerism with sperm quality and genotype can better inform the selection of chimeras for IVF to rescue GLT than coat color chimerism alone.
Research Using MBP Resources and Expertise
Please visit PubMed for more published UC Davis Mouse Biology Program research papers.
