Embryonic Stem (ES) Cell Targeting
Achieve precise genetic modifications or sequence insertions using our C57BL/6N ES cells. Our services encompass recombination, transgenesis, and non-homologous end-joining, delivering tailored solutions for your research needs.
CRISPR/Cas9-assisted gene targeting in embryonic stem (ES) cells creates custom alleles tailored to specific research objectives. These include constitutive knockouts (KO), which permanently disrupt gene function, and conditional knockouts (cKO), which allow for precise cell-specific or inducible gene inactivation. Additionally, knockin (KI) alleles are generated through targeted insertion of specific sequences, while random and targeted transgenesis introduce foreign DNA into the genome for diverse experimental applications.
Advanced techniques ensure the highest level of experimental accuracy and reproducibility. Electroporation delivers CRISPR/Cas9 components into ES cells, followed by drug selection to isolate successfully targeted cells. Rigorous genotyping and chromosome counting ensure that the correct and desired genetic modifications are maintained in clonal expansions. ES cells and genetic clones are cultured with standardized protocols to maintain pluripotency and are regularly tested for mycoplasma to maintain cell line health and quality.
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