Molecular Constructs

Advance your research with molecular construct services, providing precise design and development of genetic tools for your studies with the Mouse Biology Program.

The Mouse Biology Program provides advanced molecular construct services to support your research with precision and reliability. Using cutting-edge techniques—including CRISPR gene editing, BAC and Yeast Recombineering, Gibson Assembly Cloning, and traditional ligation—we create custom Knockout (KO), Conditional Knockout (cKO), Knockin (KI), and Transgenic (Tg) vectors. Simply provide the gene name and desired product type, and our expert team will deliver high-quality, tailored constructs to meet your experimental goals.

Consultation and Strategy Development

We begin by conducting a thorough one-on-one consultation to understand your specific research goals and objectives. Our team of experienced researchers collaborates with you to develop a tailored experimental strategy that encompasses all essential elements. This process includes evaluating potential genetic modifications, determining breeding schemes, identifying phenotyping requirements, and outlining statistical analyses to be used. By carefully considering these factors upfront, we ensure that the experimental design aligns with your research questions, maximizes accuracy, and supports reproducible results.

Customized Study Planning

Our study planning services are highly flexible, accommodating a wide range of research focuses, including disease mechanisms, therapeutic interventions, and genetic pathways. We design studies to directly address your specific research questions, implementing controls that enhance the validity of results. Our team selects the most relevant phenotyping endpoints to measure outcomes accurately and determines appropriate sample sizes to maintain statistical power and significance. This comprehensive planning approach ensures that the study delivers clear, interpretable, and actionable findings.

Phenotyping Protocol Integration

Integrating standardized and validated phenotyping protocols into your experimental design is a key component of our services. We tailor the protocols to ensure that they measure relevant biological outcomes accurately. Our assessments cover a broad spectrum, including behavioral tests for neurological function, metabolic profiling for energy balance and glucose metabolism, and cardiovascular assessments for heart rate and blood pressure monitoring. By applying these protocols consistently, we maintain the integrity and reproducibility of your experimental results.

Genetic Analysis Planning

For studies involving genetically modified models, our services include detailed planning for genotyping, gene expression analysis, and sequence validation. We guide you through each stage of genetic analysis, ensuring that modifications are accurately tracked and verified. This includes advising on the best approaches for PCR validation, RNA sequencing, or other relevant analyses, and coordinating with genetic testing laboratories to facilitate seamless data collection and interpretation.

Data Collection and Analysis Framework

We provide a comprehensive framework for data collection and analysis, ensuring that all measurements are recorded systematically and accurately. This includes setting up robust data entry systems, implementing quality checks to verify data accuracy, and selecting appropriate statistical methods to analyze the data. By ensuring a well-organized data flow, we help maintain consistency and precision in results, supporting clear interpretation and reporting.

Compliance and Ethical Considerations

Compliance with ethical guidelines and regulatory standards is a top priority in our experimental design process. We ensure that all aspects of the design meet the requirements of IACUC protocols and adhere to other animal care standards. Our team provides guidance on obtaining necessary approvals, maintaining animal welfare, and adhering to regulatory requirements throughout the study, supporting ethical and responsible research practices.

Discover Precision Tools for Advancing Your Research

With the Mouse Biology Program’s molecular construct services, you can streamline the creation of custom genetic tools tailored to your research needs. Our expertise ensures precision, efficiency, and reliable results, empowering you to focus on advancing your discoveries. Contact us today to explore how our molecular construct services can accelerate your research progress.

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