Viability Screen
To determine embryonic lethality or perinatal subviability, we conduct viability screens at key gestational stages (E9.5, E12.5, E15.5, and E18.5). Pregnant female mice undergo embryo harvesting, followed by a thorough assessment of developmental progression and viability. This service includes gene-specific genotyping of individual embryonic yolk sacs, quantitative analysis of embryo resorptions, and calculation of zygosity ratios (homozygous, heterozygous, and wild-type). The results provide critical insights into embryonic survival windows and genetic impacts on development.
Morphological Annotation
Our standardized morphological annotation process provides detailed documentation of embryo development. Using Mammalian Phenotype (MP) Ontology terms, each embryo undergoes an evaluation for structural abnormalities and developmental progression. Every embryo is meticulously examined, photographed, and annotated to ensure accurate phenotypic characterization. This standardized approach facilitates comparative analysis across multiple genetic backgrounds and experimental conditions.
Imaging
Advanced imaging techniques allow for precise visualization of embryonic structures and internal anatomy. Using high-resolution micro-computed tomography (μCT), we generate 3D reconstructions of embryos, enabling detailed morphological assessments of more than 60 different organs and tissues. These high-fidelity imaging datasets provide unparalleled insight into structural variations and developmental defects, making them invaluable for studying genetic influences on organogenesis.
Histology
Histological evaluation provides cellular-level insights into embryonic development. For embryos at E15.5 and beyond, tissues are carefully harvested, fixed, embedded, and sectioned for hematoxylin/eosin (H&E) staining. Digital imaging allows for detailed evaluation of tissue architecture and structural integrity, supporting in-depth histopathological assessments. These analyses are crucial for understanding the cellular consequences of genetic modifications and environmental influences on development.
B-Galactosidase Staining
For embryos expressing the LacZ reporter gene, we offer whole-mount β-galactosidase staining to visualize gene expression patterns during development. Embryos at E12.5 are processed to highlight specific gene activity, providing spatial and temporal expression profiles. Each stained embryo is photo-documented and annotated, creating a permanent record for downstream analyses and comparative studies.
Placenta Analysis
The placenta plays a critical role in supporting embryonic development, and our services provide comprehensive placenta analysis for embryos at E12.5 and later stages. We assess placental weights, gross morphology, and histology to identify potential abnormalities in structure or function. Placentas can be preserved in specific buffers for additional biochemical and molecular analyses, supporting investigations into fetal-maternal interactions and placental efficiency.
Special Services
Our team of highly trained technical staff and embryologists provides specialized support for customized research projects. With extensive experience in embryo harvesting, dissection, annotation, and processing, we offer tailored solutions for unique experimental needs. A dedicated embryologist is available for morphological assessments, ensuring accurate identification of normal and abnormal phenotypes. Whether conducting large-scale embryo studies or addressing specific developmental questions, our expertise enhances the precision and depth of embryological research.
