We value your privacy

We use cookies to enhance your browsing experience, serve personalized ads or content, and analyze our traffic. By clicking "Accept All", you consent to our use of cookies.

Customize Consent Preferences

We use cookies to help you navigate efficiently and perform certain functions. You will find detailed information about all cookies under each consent category below.

The cookies that are categorized as "Necessary" are stored on your browser as they are essential for enabling the basic functionalities of the site. ... 

Always Active

Necessary cookies are required to enable the basic features of this site, such as providing secure log-in or adjusting your consent preferences. These cookies do not store any personally identifiable data.

No cookies to display.

Functional cookies help perform certain functionalities like sharing the content of the website on social media platforms, collecting feedback, and other third-party features.

No cookies to display.

Analytical cookies are used to understand how visitors interact with the website. These cookies help provide information on metrics such as the number of visitors, bounce rate, traffic source, etc.

No cookies to display.

Performance cookies are used to understand and analyze the key performance indexes of the website which helps in delivering a better user experience for the visitors.

No cookies to display.

Advertisement cookies are used to provide visitors with customized advertisements based on the pages you visited previously and to analyze the effectiveness of the ad campaigns.

No cookies to display.

Embryonic Stem (ES) Cell Targeting

Achieve precise genetic modifications or sequence insertions using our C57BL/6N ES cells. Our services encompass recombination, transgenesis, and non-homologous end-joining, delivering tailored solutions for your research needs.

CRISPR/Cas9-assisted gene targeting in embryonic stem (ES) cells creates custom alleles tailored to specific research objectives. These include constitutive knockouts (KO), which permanently disrupt gene function, and conditional knockouts (cKO), which allow for precise cell-specific or inducible gene inactivation. Additionally, knockin (KI) alleles are generated through targeted insertion of specific sequences, while random and targeted transgenesis introduce foreign DNA into the genome for diverse experimental applications.

Advanced techniques ensure the highest level of experimental accuracy and reproducibility. Electroporation delivers CRISPR/Cas9 components into ES cells, followed by drug selection to isolate successfully targeted cells. Rigorous genotyping and chromosome counting ensure that the correct and desired genetic modifications are maintained in clonal expansions. ES cells and genetic clones are cultured with standardized protocols to maintain pluripotency and are regularly tested for mycoplasma to maintain cell line health and quality.

Order ES Cell Targeting Service
Design Your Mouse
Manage Your Mouse
Study Your Mouse

Not seeing something you need? Contact Us