Genetic Analysis Services

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The Mouse Biology Program offers a suite of genetic analysis services to confirm and characterize mouse models at the DNA level. Our services, including Allele-Specific PCR, Sanger Sequencing, and Loss-of-Allele Analysis, provide precise genotyping and verification for newly created and existing models.

Allele-Specific PCR

Allele-Specific PCR is a powerful technique for detecting specific alleles in a gene, including point mutations, knockouts, and knockins. This method uses primers to amplify only the target allele, enabling rapid and accurate identification.

• Applications:
  • Identifying specific gene modifications (e.g., knockouts, knockins).
  • Differentiating between wild-type, heterozygous, and homozygous genotypes.
  • Screening for point mutations or small insertions/deletions.
• Advantages:
  • Fast and cost-effective.
  • High specificity for detecting genetic variations.
  • Useful for routine genotyping and breeding confirmation.

Sanger Sequencing

Sanger Sequencing is the gold standard for DNA sequencing, providing accurate base-by-base confirmation of genetic modifications. This method is particularly useful for validating CRISPR edits, verifying gene sequences, and confirming the integrity of targeted mutations.

• Applications:
  • Confirming precise nucleotide changes introduced by gene editing.
  • Validating the sequence of transgenic constructs.
  • Detecting off-target effects in modified strains.
• Advantages:
  • High accuracy in detecting single-nucleotide changes.
  • Suitable for sequencing specific regions of interest.
  • Reliable for both small and large genetic modifications.

Loss-of-Allele Analysis

Loss-of-allele analysis is critical for assessing whether a specific allele has been successfully knocked out in a mouse model. This technique is commonly used with allele-specific PCR and sequencing to ensure complete loss of the target allele.

• Applications:
  • Confirming successful gene knockout in mouse models.
  • Assessing genetic changes in complex breeding strategies.
  • Supporting research on gene function by verifying complete loss of function.
• Advantages:
  • Provides explicit confirmation of allele loss.
  • Can be integrated with other genetic analysis methods for comprehensive validation.
  • Essential for verifying knockout efficiency and genetic integrity.

Accurate Genotyping for Reliable Research Outcomes
Our genetic analysis services are designed to provide researchers with precise, reliable, and reproducible results. Whether you are validating a newly created model or confirming breeding outcomes, our advanced genotyping methods support your research at every stage.

For more information or to request these services, don’t hesitate to contact our team.

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• Genotyping Services
• Coding Sequence Validation
• Genetic Analysis Services
• Genomic Insertion Site Identification
• Homologous Recombination Confirmation in ES Cells
• Off-Target Mutagenesis Detection