Genomic Insertion Site Identification

---

Genomic insertion site identification is essential for confirming the exact location of DNA insertions within the mouse genome, such as transgenes or randomly integrated constructs. This process ensures that modified models function as expected and helps researchers understand any positional effects that might influence gene expression.

What is Genomic Insertion Site Identification?

This analysis involves determining where foreign DNA has integrated into the genome. It is vital in validating transgenic models, characterizing gene functions, and assessing the effects of targeted and random DNA integration.

Accurate mapping of DNA integration sites is crucial for characterizing mouse models and driving reliable, impactful research.

How We Identify Genomic Insertion Sites

• Inverse PCR (qPCR):
  Inverse PCR amplifies genomic regions flanking the inserted DNA, enabling the detection of specific integration sites. This method is effective for both targeted and random insertions.
• Next-Generation Sequencing (NGS):
  NGS provides high-resolution mapping of the exact insertion site and junction sequences. It also offers insights into potential off-target integrations or structural changes at the insertion site.
• Southern Blot Analysis:
  For additional validation, Southern blotting detects the presence, copy number, and integrity of inserted sequences, ensuring the model’s stability and accuracy.

Why is Genomic Insertion Site Identification Important?

• Assessing Positional Effects:
  The integration site can impact gene expression, regulatory elements, and overall genomic stability, influencing the modified model’s phenotype.
• Validating Transgenic Models:
  Confirming insertion location ensures that genetic modifications have occurred as intended, supporting the reliability of transgenic research.
• Detecting Unintended Insertions:
  Identifying off-target insertions prevents confounding results, enabling more accurate experimental outcomes and phenotypic interpretations.

How to Request Genomic Insertion Site Identification

If you need genomic insertion site identification, please contact our team. We guide sample submission, analysis, and results interpretation to ensure your genomic insertion projects meet the highest standards.

---

• Genotyping Services
• Coding Sequence Validation
• Genetic Analysis Services
• Genomic Insertion Site Identification
• Homologous Recombination Confirmation in ES Cells
• Off-Target Mutagenesis Detection