Embryo Cryopreservation Service

 

Cryopreservation of embryos defines the long-term preservation of the preimplantation embryos which suspends its metabolism and development by freezing and storing them in liquid nitrogen. Should the need arise, the cryopreserved embryos can be thawed and implanted into pseudopregnant females in order to produce pups which can be bred to establish a colony.

Mouse embryos at 2-cell stage derived from IVF are cryopreserved using PROH (1,2-Propanediol) as cryoprotectant (Renard and Babinet, 1984) and a programmable freezer. Our cryopreservation lab attempts to preserve 250-300 embryos (20-25 embryos per straw) per strain using two IVF attempts. Some mutant strains cannot provide that number of embryos from two IVF procedures, and additional charges may be incurred if additional attempts are required.

 

Heterozygous Line Embryo Cryopreservation

For heterozygous line embryo cryopreservation, we prefer to receive 2-3 males from our customer, and we will purchase the female mice as egg donors for IVF (in vitro fertilization). 

 

Homozygous Line Embryo Cryopreservation

For homozygous line embryo cryopreservation, we like to receive 2-3 males and at least 10-15 mutant females per line.

The optimal male age is 11-20 weeks old, and the optimal female age is 3-8 weeks old. The fee for embryo cryopreservation includes up to two IVF procedures, cryoarchiving up to 250-300 embryos, and the storage of the cryopreserved materials for one year.

 

Please see our order page for additional information on our cryopreservation services.

 

Reference:

Renard JP, Babinet C 1984 High survival of mouse embryos after rapid freezing and thawing inside plastic straws with 1–2 propanediol as cryoprotectant. Journal of Experimental Zoology 230: 443–448.

 

 

 

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