Digital Resource Library

At the heart of the Mouse Biology Program is research and education.  Below is our digital resource library where we host links and downloads about our published research, available services and educational material. 

Published Research

MBP Research

Conditional targeting of Ispd using paired Cas9 nickase and a single DNA template in mice

Angus Yiu-fai Lee and Kevin C. Kent Lloyd

In this study, Cas9 RNA and  sgRNAs were injected together with a single DNA template encoding two loxP sites flanking (floxing) exon 2 of the isoprenoid synthase containing domain (Ispd) into the pronucleus and cytoplasm of C57BL/6NCr one-cell stage zygotes. After surgical transfer, one F0 mouse expressing a conditional allele was produced (at a frequency of ∼8% of live pups born). The floxed allele was transmitted through the germline to F1 progeny and could be successfully recombined using Cre recombinase. This study indicates that conditional targeting can be accomplished effectively using paired Cas9n and a single DNA template.

Rescue of germline transmission from chimeras by IVF after sperm analysis

Li MW, Willis BJ, Evans KD, Araiza RS, Lee AY, Lloyd KC.

In this  study we assessed the production, quality and genotype of epididymal sperm harvested from male chimeras generated by blastocyst or morula microinjection of gene targeted embryonic stem (ES) cell clones containing a LacZ expression cassette and that failed to achieve GLT. Our results indicate that most chimeras that do not achieve GLT by natural mating are infertile, and the primary cause of infertility is failed spermatogenesis. We conclude that an assessment of coat color chimerism together with sperm quality and genotype can better inform the selection of chimeras for IVF to rescue GLT than coat color chimerism alone.

Research Using MBP Resources and Expertise

Please visit PubMed for more published research papers from UC Davis Mouse Biology Program.