Strain rederivation is a management tool used to clean a mouse line from pathogen infection or to import mice into a barrier facility from an outside vivarium. We offer rederivation service by IVF or ICSI followed by embryo transfer into pseudopregnant recipients to produce pups in a barrier facility.
If your mouse colonies have ceased reproducing or are threatened by health or other issues we can also help. We offer strain rescue service by IVF or ICSI followed by embryo transfer to produce pups in a barrier facility.
We highly recommend adding sperm cryopreservation services of the mutant male(s) being used for rederivation or rescue. Embryo cryopreservation is also recommended if extra embryos are produced by IVF.
Please see our order page for additional information on rederivation pricing.
Does your project require dozens or hundreds of mice? If your project requires a lot of pathogen-free mice at the same age, our speed expansion service can help you. We do IVF to produce plenty of embryos for embryo transfers into enough pseudopregnant recipients to produce pups at the same age. This saves months when compared to traditional breeding approaches and requires only one or two male mice.
Please see our order page for additional information on expansion service pricing.
Li MW, Willis BJ, Lloyd KCK, et al. (2015) Rescue of germline transmission from chimeras by IVF after sperm analysis. Transgenic Res. 24: 99-108.
Li MW, KCK Lloyd, et al. (2014) IVF recovery of mutant mouse strains using sperm cryopreserved with MTG in cryovials. Cyro Letters 35: 145-153.
Li MW, Lloyd KCK, et al. (2013) Safety, efficacy and efficiency of laser-assisted IVF in subfertile mutant mouse strains. Reproduction 145: 245-54.
Li MW, Lloyd KCK (2006) Intracytoplasmic sperm injection (ICSI) in the mouse. In “Principles and Practice: Mammalian and Avian Transgenesis – New Approaches”, Shirley Pease and Carlos Lois (Eds), Springer–Verlag Berlin Heidelberg, p23-40