To better serve the research community, we have developed a convenient ready to use In Vitro Fertilization (IVF) kit available online. This kit is based on our extensive in house research and according to our high quality control IVF protocols. Each kit contains 4x10 ml IVF medium with high calcium, 2x ≥ 1 ml MBCD medium and 0.0225 g GSH (L-glutathione reduced), enough for at least two IVF procedures. This IVF Kit may be used for IVF using either fresh or frozen-thawed sperm.
Li MW, Vallelunga JM, Kinchen KL, Rink KL, Zarrabi J, Shamamian A, Glass OC, Lloyd KCK 2014 IVF recovery of mutant mouse lines using sperm cryopreserved with MTG in cryovials. Cryo Letters 35:145-153.