Knockouts & Knockins

-ES Cell Targeting-

 

The Mouse Biology Program (MBP) offers gene targeting by homologous recombination in embryonic stem (ES) cells to create a diversity of allele types, including constitutive knockout (KO), conditional (cell specific and/or inducible) knockout (cKO), knockin (KI), and targeted transgenic (TT) for your specific gene of interest.  The MBP also offers guaranteed germline transmission (gGLT) from injection of gene-targeted ES cells using an MBP-designed targeting vector.

 

The MBP is one of the very few programs in the world that offers guaranteed germline transmission from its own gene targeted ES cells on a C57BL/6 genetic background. To take advantage of Guaranteed Germline Transmission, request MBP to design and construct a targeting vector, perform ES cell electroporation, and microinject gene-targeted ES cells.  We will deliver at least two, genotype-confirmed heterozygous mutant mice. MBP offers a wide array of products and custom research services including engineered nuclease technologies (CRISPR/Cas9) to create knockouts, transgenic mouse line creationembryo and sperm cryopreservationreanimation and rederivationgenotyping and phenotyping, custom colony management services and much more.

 

ES cell gene targeting includes:

 

 

 Targeted Allele Types:

 

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