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MBP Products & Services                          Service Description                                     
     
UC Recharge Rate*

Non-UC Academic   Rate**

 Industry Rate**
Molecular Biology                                                                                                                                      
Vector Construction  Dependent on project requirements, please contact us for details. contact us for details    
Targeting vector verification (KOMP/EUCOMM)  DNA isolation and QC by restriction digest and preparation  for electroporation. DOES NOT INCLUDE VECTOR PURCHASE.
$1,605 $2,324.04  $2,484.54
Transgenic Vector purification (BAC/High copy plasmid) Linearization and/or Purification of DNA for Pronuclear injection to create transgenic founders.
$1068/$848 $1546.46/$1227.90  $1653.26/$1312.70
CRISPR guides and/or oligo design, construction, synthesis
 Dependent on project requirements, please contact us for details. contact us for details    
         
Embryonic Stem (ES) Cells        
Electroporation

Electroporation, selection and picking of up to 3 X 96 clones, expansion of       up to 12 potential positives

$4,240 $6,139.52  $6,563.52
Chromosome Counting (karyotyping) Cells are grown, metaphase spreads prepared, and digital images of spreads     are analyzed to determine the number of chromosomes per spread, for a minimum of 20 spreads. $266 $385.17 $411.77
Clone expansion When only a single vial of cells ( e.g., EUCOMM or outside targeting lab) is available and  back-up vials are needed. In general 6-8 vials are frozen back/per clone $256 $370.69  $369.29
FLPe/Cre electroporation (e.g., excise Neo) Vector preparation, electroporation and, picking of up to 96 clones $3,280 $4,749.44  $5077.44
         
Genotyping        
Standard PCR Protocol Development PCR protocol development per targeted allele.  $483 $699.38  $747.68
Mouse Colony Genotyping  (e.g.standard PCR) per sample $13 $18.82  $20.12
ES Cell screening (PCR) Screening of up to 3x 96 well plates for Homologous recombination               (LOA, LR PCR, Copy#, Loxp check)
$2,776 $4,019.65  $4,297.25
Speed Congenics (SNP) per sample $151 $218.65  $233.75
Taqman qPCR gene Expression Dependent on project requirements, please contact us for details contact us for details    
Rapid Amplification of cDNA Ends (RACE) per clone $285 $412.68  $441.18
Vector Integration Site Analysis (VISA) per clone, dependent on gene trap intron size, please contact us for details contact us for details    
Southern Blot Genotyping Confirmation per targeting arm--up to 18 samples with the same NEO probe                (expansion of ES cells not included) $1,602 $2,319.70  $2,479.90
Next-Generation Sequencing Dependent on project requirements, please contact us for details contact us for details    
         
Microinjection (genetic background)        
CRISPR-Cas9 zygote injection (C57BL/6) 180 embryos microinjected and transferred. Up to 40 pups produced.       Housing of born pups until 10 weeks of age. May require two injection sessions for guide plus construct.
$4,654.15 $6,739.20  $7,204.62
Transgenic (C57BL/6)-Pro-nuclear injection, Basic
180 embryos microinjected and transferred. Up to 40 pups produced.      Housing of born pups until 9 weeks of age. May require 2x injection sessions for BAC constructs.
$4,869.59 $7,051.17 $7,538.13
Transgenic (C57BL/6)-Pro-nuclear injection, Guaranteed Ask about options for guaranteed production of transgenic founders.
contact us for details    
Knockout‐ES Cell injection to blastocyst or morula  (Inbred)

Basic Microinjection Services includes microinjection of up to 3 ES cell clones per targeted allele, with injection of at least 30 blastocysts or embryos per clone, derivation of 8 pups or 1 appropriately-sexed chimera per targeted allele with ≥50% ES-cell derived coat color, whichever comes first, and housing of pups until 7 weeks of age. While we will make every reasonable effort, we cannot guarantee derivation of high percent chimeric mice with basic microinjection services. ES cells can be requested for shipment to your lab (additional fees may apply).

$6,644.26 $9,620.89  $10,285.31
Knockout - ES Cell injection Guaranteed Chimera‐blastocyst, morula (Inbred) We are pleased to offer Guaranteed Microinjection Services on a limited number of our ES cell lines. This service includes microinjection of up to 3 ES cell clones per targeted allele, with a guarantee to produce at least 2 appropriately-sexed chimeras per targeted allele with equal to or greater than 50% ES-cell derived coat color. ES cells can be requested for shipment to your lab (additional fees will apply). Refund applies only to the microinjection service fees, all other fees for services provided will apply $7,628.71 $11,046.37  $11,809.25
         
Vivarium Services        
Colony Management (per cage/day) per cage/day, communications,  breeding, sampling, culling, and other husbandry requirements
$0.53 $0.77  $0.83
Per Diem (Modified Barrier/Barrier housing) per cage/day $1.23/$1.85 $1.78/$2.67  $1.90/$2.86
Delivery/Vehicle fee
Delivery of mice or material between facilities.
$76  $110.05  $117.65
Cesarean Rederivation
Rederivation of a mouse line via cesarean, from importation to 2nd St. conventional housing.  Cannot be used for rederivation to the M3 barrier facility.
$1,198.54  $1,735.49  $1,855.34
Germline Transmission Testing Dependent on project requirements, please contact us for details contact us for details    
         
Cryopreservation        
Sperm Cryopreservation Sperm cryopreservation for up to 2 male mice. Sperm counts, motility and      morphology  are analyzed prior to cryopreservation and the sperm               quality assessment is provided to you upon completion. 1 year of                  storage fee included. $468.05 $677.74  $724.55
Embryo Cryopreservation Heterozygous line, includes up to two IVF procedures, cryoarchiving  up to 300 embryos. 1 year of storage fee included.  Homozygous line fees will vary, and may require additional breeding and cryo sessions, please contact us for more info.
$2,347.11 $3,398.62  $3,633.33
IVF Kit Each IVF kit is enough for 2 IVF procedures. An IVF kit is good for use              for up to three months if kept at -20°C or -80°C; once thawed it is good             for two weeks if refrigerated. $284 $411.23  $439.63
         
Rederivation & CryoRecovery        
Rederivation (to liveborn offspring) Up to two IVF procedures, embryo transfers into pseudo pregnant         recipients, and housing of born mice for up to 7 weeks. $3,878.20 $5,615.64  $6,003.46
Sperm Resuscitation (IVF/ICSI) Up to two IVF procedures, embryo transfers into pseudo pregnant          recipients, and housing of born mice for up to 7 weeks. If necessary,                ISCI may be performed

$3998.43/$4664.99

$5789.73/$6754.90

 $6189.57/ $7221.40

Embryo Resuscitation Embryo transfers into pseudo pregnant recipients, and housing of born           mice for up to 7 weeks $2,664.21 $3,878.20  $4,124.20
         
Import & Export Services        
Import Mice or Material Processing fees for importation of mice and/or material, including shipment coordination, animal health approvals, vivarium coordination.
$148 $214.30  $229.10
Export Mice (domestic/international)
Processing fees for exportation of mice, including shipment coordination, documentation.  Does not include courier fees or mouse crates (prices below).
 $114/$228  $165.07/$330.14  $176.47/$352.94
Mouse Crate - Small Includes sterilized 1 - 2 compartment mouse crate, bedding, nectar gel and rodent chow for domestic and international transport, packing, and transfer to shipping area. $86 $124.53 $133.13
Mouse Crate - Large Includes sterilized 1 - 4 compartment mouse crate, bedding, nectar gel and rodent chow for domestic and international transport, packing, and transfer to shipping area. $129 $186.79 $199.69
Export Material in an Insulated Box Domestic/International. Includes handling, processing, box, dry or wet ice as needed.  Does not include courier fees. $68/$189 $98.46/$273.67 $105.26/$292.57
Export Cryopreserved material (domestic/international)
Includes use of vapor shipper use, liquid nitrogen charging of shipper shipment handling and coordination.  Our vapor shippers are routinely checked and tested for quality assurance and control.  $100/$214 $144.80/$309.87 $154.80/$331.27
         
Phenotyping Services
       
Perfusions, Analyses, Xenograft, PDX, Embryo Collections, Behavioural Testing, and other in vitro and in vivo services.                                  
Dependent on project requirements, please contact us for details. contact us for details    
         
         

 

The University of California, Davis, Mouse Biology Program (MBP) is a coordinated, campus-supported teaching, research, and service resource of infrastructure and fundamental expertise directed at developing, promoting, and enhancing the use of mouse model systems for understanding and resolving biological problems. Service rates listed here are university approved and subject to change.

If eligible, UC Davis Cancer Center members may receive a discount on Cancer related research. 

Prices listed subject to change (last update 7/7/2016). Request a quote from MBP Customer Service (This email address is being protected from spambots. You need JavaScript enabled to view it.) for your project.

* Payment must be made from a UC account. Shipping, order processing and crate or box fees are extra.

**Invoices will be emailed to the client. Shipping, order processing and crate or box fees are extra.

 

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